Application

Immunocytochemistry

ELISA

Stimulation/Inhibition of biological activity

Electrophoretic mobility shift analysis

Optimal working dilutions must be determined by end user.

Detect CD4 using this Anti-CD4 Antibody, intracellular, clone 024-10D6.B3 validated for use in ELISA, EMSA, FUNC & IC.

Immunogen

Synthetic peptide corresponding to amino acids 439-458 of the cytoplasmic tail of human CD4.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin. Liquid in 0.02 M phosphate buffer, 0.25M NaCl, with 0.1% sodium azide.

Format: Purified

Specificity

Recognizes the cytoplasmic tail of CD4. Many functions have been assigned to this tail, including anti-viral regulation (down/up regulation of CD4), T-cell maturation, activation and differentiation, and Lck signal transduction, among others. This antibody is useful as a control for studies involving targeting of intracellular protein domains of T cells.